nprB [2017-8-11 16:0:3]

extracellular neutral protease B

locus: BSU11100

pI: 5.32

mw: 59.17 kDa

protein length: 538 aa

gene length: 1614 bp

function: degradation of proteins

product: extracellular neutral protease B

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 1,186,037 → 1,187,653

The protein

Catalyzed reaction/ biological activity

• Similar, but not identical, to that of thermolysin (according to Swiss-Prot)

Protein family

• peptidase M4 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|309EAC680ABFF0B736E01F0B9BDFBB2FCBDB1BBF|NprE]

Structure

• [PDB|5A3Y] (thermolysin, 44% identity) [pubmed|26527148]

[SW|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|57139471EB143F6DCCF092DAD0FBB43B0D50D948|nprB]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [pubmed|31622331], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]

  regulation

  • induced in biofilms ([SW|DegU]) [Pubmed|31622331]

Biological materials

Mutant

• BKE11100 (''ΔnprB::erm trpC2''), available at BGSC [pubmed|28189581]
• KO7 (''ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr''), available as BGSC 1A1133

References

Reviews

• 20735481

Original publications

• 24115457,26527148